The cartulary is a volume of 90 parchment folios. It was produced in the middle of the period of the the Hospital's greatest territorial expansion: a majority of the deeds included record transactions between c.1220 and c.1280; also includes a folio of kitchen accounts from 1344.

Includes: the 1921 report on the College Archives and Records and the 1933 report on the College muniments with respect to the repair of the Cartulary of the Hospital of St John with related correspondence.